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Regulator of G protein Signaling 3 Modulates Wnt5b Calcium Dynamics and Somite Patterning
Author(s) -
Freisinger Christina M,
Slusarski Diane C
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.451.4
Subject(s) - regulator of g protein signaling , wnt signaling pathway , zebrafish , microbiology and biotechnology , context (archaeology) , biology , gtpase activating protein , g protein , regulator , gtpase , signal transduction , calcium signaling , genetics , gene , paleontology
We have identified Ca 2+ release as an output of Wnt signaling network and are investigating the mechanisms and context by which this Wnt signal mediates its biological effect on the developing embryo. We have determined that the early Ca 2+ transients are, in part, modulated by Wnt‐5 and require G‐protein signaling. To identify potential intracellular regulators of calcium release dynamics, we are characterizing Rgs3, a member of the Regulators of G‐protein Signaling (RGS) protein family. This work integrates molecular, genetic and physiological approaches to investigate the mechanism and context mediating calcium release dynamics and the biological effect on developing zebrafish embryos in vivo. Using loss of function analysis, we demonstrate that Rgs3 is necessary for proper gastrulation and somite patterning during zebrafish development. These actions of Rgs3 require its ability to interact with and accelerate the rate of GTP hydrolysis by G proteins, as revealed by studies employing an Rgs3 mutant defective in these activities. This study identifies a previously unknown genetic interaction between RGS and the non‐canonical Wnt signaling network. Grant Funding Source : March of Dimes (5‐FY99‐806); NIH (RO3 HD045488‐01 ), RO1 ( GM067881‐01 ); and American Heart Association Predoctoral Fellowship