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Genomic approaches for enhancer identification
Author(s) -
Rubin Edward M.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.414.2
Subject(s) - enhancer , computational biology , biology , identification (biology) , genomics , chromatin , genome , enhancer rnas , genetics , gene , human genome , transcription factor , botany
Despite their anticipated importance in human biology and disease, transcriptional enhancers remain challenging to both identify as well as predict their associated in vivo gene regulatory activities. I will describe two whole genome strategies for the identification of Mendelian enhancers, comparative genomics and Chromatin Immunoparticipation coupled with massively parallel next generation sequencing (ChiP‐Seq). Both of these enhancer prediction approaches have been linked to large scale in vivo validation where ~1500 putative enhancers have been tested in a transgenic mouse enhancer assay system. Summaries of the results and insights will be presented.