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Genome‐wide DNA methylation profile of pluripotent stem cells
Author(s) -
Sato Shinya,
Yagi Shintaro,
Shiota Kunio
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.39.9
Subject(s) - dna methylation , biology , induced pluripotent stem cell , epigenetics , differentially methylated regions , methylation , genetics , gene , embryonic stem cell , gene expression
DNA methylation is a vital mark for epigenetic regulation of gene expression. Here, we profiled geneome‐wide DNA methylation status in mouse pluripotent stem cells, focusing on tissue‐dependent and differentially methylated regions (T‐DMRs) of ESCs. By using T‐DMR profiling with restriction tag‐mediated amplification (D‐REAM), we identified thousands of both hypo‐ and hypermethytaed T‐DMRs characterizing the pluripotent cells. The hypomethylated T‐DMRs were found in genes involved in nuclear events as transcriptional regulation network of ESCs, and majority of them are in genes associated with CpG Islands. In addition, hypermethylatied T‐DMRs are found in genes for tissue‐specific function. Based on the T‐DMRs, we further introduce the commonality of DNA methylation profile between pluripotent stem cell lines. Our results indicate that DNA methylation profile contribute to the mechanism of pluripotency, and can make novel criteria for evaluation and standardization of pluripotent stem cells. This work is supported by the grants from the National Institute of Biomedical Innovation and the New Energy and Industrial Technology Development Organization, Japan.