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Voltage‐dependent K+ channels in Immunogenic and Regulatory Functions of Lung Dendritic Cells
Author(s) -
Wang Xiang,
Shao Zhifei,
Agrawal Devendra K.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.355.8
Subject(s) - cd11c , microbiology and biotechnology , immune system , cd86 , chemistry , dendritic cell , integrin alpha m , chemokine , ion channel , immunology , biology , t cell , biophysics , receptor , biochemistry , phenotype , gene
Dendritic cell (DC) subsets display different functional roles in regulating immune responses. We identified two DC populations, CD11c low CD11b high and CD11c high CD11b low , in the lungs of asthmatic mice. The DC subsets show distinct expression patterns in their surface markers and chemokine receptors. Since K + channels modulate the function of T‐lymphocytes and mast cells, we examined ion channel expression of both DC subsets using whole‐cell voltage‐clamp techniques. At least two types of voltage‐dependent K + currents were detected in both DCs, demonstrated by differential inhibitory effects of the selective Kv1.3 blocker, margatoxin, and the non‐selective voltage‐sensitive Ca 2+ ‐activated K + channel blocker, charybdotoxin. Voltage‐dependent proton currents were also detected in both DCs, and this was blocked by 10 mM Zn + . This suggests the potential role of lung DCs in the phagocytosis through activating the NADPH oxidase. The CD11c low CD11b high DCs exhibited significantly larger amplitudes in all current than CD11c high CD11b low DCs ( p <0.01), suggesting that CD11c low CD11b high DCs possess a higher ion channel expression level in the membrane. This differential expression level of ion channels may shed light on the distinct functional properties between the two DC subsets and their migration to the lymph node during allergic immune response. (Supported by LB506 DHHS State of Nebraska cancer and Smoking‐related Disease Program)