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Regional localization of intestinal dendritic cell subsets control Th‐17 responses
Author(s) -
Denning Tim,
Norris Brian,
Greene Brent,
Pulendran Bali
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.355.7
Subject(s) - cd11c , integrin alpha m , flow cytometry , foxp3 , t cell , small intestine , microbiology and biotechnology , biology , chemistry , immunology , immune system , biochemistry , phenotype , gene
Mucosal immunity is influenced in large part by the dynamic interplay between various intestinal antigen‐presenting cell subsets and T lymphocytes. Using 10‐color flow cytometry, we have defined the major DC and macrophage subsets in the small and large intestine LP as distinguishable by the reciprocal expression of CD103 and F4/80, respectively, and not exclusively by CD11b or CD11c. Intestinal F4/80 + macrophages expressed IL‐10, TGF‐β, retinoic acid‐generating enzymes and promoted the differentiation of inducible FoxP3 + regulatory T cells. Alternatively, CD11b + , but not CD11b − , CD103 + LP DCs expressed IL‐6 and TGF‐β as well as retinoic acid‐generating enzymes and efficiently induced the differentiation of T H ‐17 cells. These CD103 + LP DC subsets displayed striking region‐specific localization along the intestine with CD11b − CD103 + LP DCs appearing most abundantly in the large intestine while CD11b + CD103 + LP DCs were most prevalent in the upper small intestine. The distribution pattern of CD11b + CD103 + LP DCs in particular correlated with the representation of T H ‐17 cells in the intestine and during intestinal inflammation CD11b + CD103 + LP DCs accumulated in the colonic LP as did T H ‐17 cells. Our results suggest that CD11b + CD103 + LP DCs may be targets for modulating IL‐17 producing T cell responses during intestinal inflammation.