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Induction of Apoptosis by Methanol Extract of Cnidium officinale Makino through Activation of Caspase‐3 and p53 in Human Liver cancer HepG2 cell
Author(s) -
An JeongCheol,
Hong HeeOk,
Hwang SeongGu
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.349.8
Subject(s) - apoptosis , chemistry , western blot , caspase , cell growth , caspase 3 , antioxidant , cell , cancer cell , pharmacology , traditional medicine , dpph , liver cancer , microbiology and biotechnology , biochemistry , cancer , programmed cell death , biology , medicine , gene
In the present study, we investigated if a methanol extract of Cnidium officinale Makino (CO) can induce the apoptosis against human liver cancer HepG2 cell. HepG2 cells were treated with medium containing 0, 0.1, 0.25, 0.5, 0.75, 1 mg/ml of CO methanol extract. Cell proliferation assay was undertaken after 24hrs of incubation. Caspase‐3 and p53 mRNA expression were measured by RT‐PCR and protein expression by Western Blot. The antioxidant activity of CO was analyzed using DPPH assay. The HepG2 proliferation was inhibited by CO extract in dose dependent manner. The CO promoted activation of apoptotic caspase‐3 and p53 genes. The CO showed free radical scavenging activity dose‐dependently. The results suggested that CO‐induced apoptosis of HepG2 cells may be mediated through the activation of apoptotic pathway involving caspase‐3 and p53. In summary, CO may provide a new therapeutic option as a potential anticancer agent in the treatment of liver cancer.