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The effects of dietary antioxidants and age on hepatic CRP levels in rodents
Author(s) -
Hasan Syeda Tahira,
Kong Eun Hee,
Guo Weimin,
Wu Dayong,
Wang YanLee,
Veeramachaneni Sudipta,
Wang XiangDong,
Meydani Mohsen
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.342.7
Subject(s) - lycopene , medicine , inflammation , c reactive protein , endocrinology , alcohol , liquid diet , fat soluble vitamin , tocopherol , antioxidant , vitamin e , chemistry , vitamin , biochemistry
Dietary antioxidants may counter inflammation associated with many diseases like CVD and cancer. C‐reactive protein (CRP) is a well‐established marker of systemic inflammation. Using ELISA, we examined CRP levels in liver samples of young and old animals and animals fed with high fat diet or alcohol diet supplemented with antioxidants. Rats treated with high fat liquid (LDC) diet (71% energy from fat) for 6 wks had NASH and increased hepatic CRP levels by 53%, as compared to control (35% energy from fat). Rats fed with liquid (LDC) alcohol diet (36% energy from alcohol) with lycopene supplementation (3.3 mg/Kg BW/d) for 11 wks increased hepatic CRP levels by 2 fold (56.8 ± 22.9 vs. 28.0 ± 7.2 pg/μg protein), as compared with rats fed control diet supplemented with lycopene. In addition, 26 mo old C57BL/6 mice had CRP levels 2.3 fold higher than young (6 mo) mice. Supplementing mice with a mixture of tocopherol/tocotrienol (provided 500 ppm vit. E/kg diet) for 6 wks significantly reduced CRP levels by 30% and 40% in young and old mice, respectively. We conclude that hepatic CRP increase with age and with consumption of high fat diet. While a mixture of tocopherol appears to attenuate CRP levels, high dose of lycopene supplementation augments hepatic CRP in livers of rats fed alcohol. The latter indicates potentially harmful effects of consuming high amounts of both alcohol and lycopene. Supported by USDA agreement # 58‐1950‐7‐707