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Persistence of Lactobacillus Reuteri DSM17938 in the Human Intestinal Tract
Author(s) -
Smith Tracey J,
Anderson Danielle,
Sikes Anthony,
Margolis Lee M,
Young Andrew J.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.340.1
Subject(s) - lactobacillus reuteri , dosing , persistence (discontinuity) , probiotic , colonization , feces , medicine , gastrointestinal tract , gastroenterology , zoology , biology , microbiology and biotechnology , bacteria , genetics , geotechnical engineering , engineering
Probiotics may enhance gastrointestinal health and immune function. The efficacy of different dosing strategies on colonization and persistence of probiotics are undefined. We assessed colonization and persistence of Lactobacillus reuteri ( L. reuteri ) (BioGaia, Stockholm, Sweden) after daily or alternate day dosing. Volunteers ate pudding with L. reuteri (10 9 CFU) daily (DAILY; n = 9) or on alternate days (ALT; n = 9) over 7 days. Fecal samples were collected on dosing days (D1–7) and after dosing ended (D13–15 and D20–22), and analyzed for L. reuteri . Results are reported in 3‐day increments (D2–4, D5–7, D13–15, and D20–22). L. reuteri count rose in DAILY ([mean ± SD] D2–4: 4 × 10 4 ± 2 × 10 4 CFU, p < 0.01; D5–7: 10 × 10 4 ± 9 × 10 4 CFU, p < 0.01) and ALT (D2–4: 21 × 10 4 ± 20 × 10 4 CFU, p < 0.01; D5–7: 11 × 10 4 ± 15 × 10 4 CFU, P = 0.06), and fell in both groups 1 week after dosing ended (p < 0.01). Total volunteers with detectable L. reuteri 1 and 2 weeks after dosing ended was similar for DAILY (4/9 and 2/9, respectively) and ALT (3/9 and 2/9, respectively). L. reuteri count was higher D2–4 for ALT vs. DAILY (p < 0.05), but similar thereafter. Alternate day probiotic intake achieves equivalent colonization to daily intake, but colonization declines rapidly once dosing stops. Initially, it appears that responsiveness to probiotics may differ between individuals, but those differences do not persist with longer consumption. Funded by MRMC.