Premium
Reduced weight gain and improved insulin sensitivity in high‐fat fed mice by treatment with a mixture of a reduced isohumulones from hops and an extract from acacia
Author(s) -
Tripp Matthew L,
Vroegrijk Irene,
Diepen Janna A,
Darland Gary,
Konda Veera R,
Bland Jeffrey S,
Voshol Peter J
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.335.4
Subject(s) - endocrinology , medicine , insulin , lipogenesis , weight gain , insulin sensitivity , insulin tolerance test , adipocyte , chemistry , glucose tolerance test , obesity , insulin resistance , adipose tissue , body weight
Bioactive natural products obtained from foods may be safely incorporated into a lifestyle program and help mitigate metabolic disturbances caused by a westernized high fat diet. Previously we screened 200+ botanicals and discovered that a mixture of an extract of Acacia nilotica (ANE) and reduced iso‐alpha acids (RHO) from Humulus lupulus increased insulin sensitivity in a 3T3‐L1 adipocyte model of lipogenesis. Subsequent studies in db/db mice established that META352, a 5:1 mixture of RHO:ANE, was optimal for reducing fasting insulin and glucose. We evaluated META352 in a model of high fat diet (HFD) induced obesity in which C57BL mice were maintained on a diet that included 45% lard for 12 weeks. HFD fed mice supplemented with META352 (100 mg/kg body weight) exhibited a significant reduction in weight gain (~20%); subcutaneous and gonadal fat were reduced by 50% and fasting glucose and insulin were reduced by 25% and 80%, respectively (p<0.05). Oral glucose tolerance test and insulin sensitivity test showed that META352 improved insulin sensitivity in the META352 supplemented mice (p<0.05) compared to the HFD fed mice. In conclusion, META352, a proprietary combination of RHO and ANE, reduced elevated plasma insulin and glucose in the db/db mice, reduced weight gain, body fat, and improved insulin sensitivity in high‐fat fed mice. Supported by MetaProteomics, LLC.