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Isoflavone‐free soy protein diet inhibits LPS‐induced inflammatory responses
Author(s) -
Goodwin Van B,
Greenway Amy,
Nagarajan Shanmugam
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.326.3
Subject(s) - inflammation , soy protein , casein , chemistry , western blot , aorta , endocrinology , medicine , biology , biochemistry , gene
Recently, we showed reduced atherosclerotic lesions in a hyperlipidemic mouse model fed isoflavone‐free soy protein diet (SPI − ) compared to casein (CAS)‐fed mice, despite unchanged serum lipid levels. However, the molecular mechanisms contributing to the atheroprotective effect of soy‐based diets is not clear. Atherosclerosis is a chronic inflammatory disease and interactions between endothelial cells and monocytes play a pivotal role in the initiation of atherosclerosis. We hypothesize that soy protein/peptides (SPI − ) exert their protective effect by inhibiting inflammatory responses contributing to both acute and chronic inflammatory conditions. To address this hypothesis, a LPS‐induced acute inflammation model in apoE−/− mouse was developed. Dose response studies showed LPS at 20 μg/mouse is sufficient to induce CD54 and CD106, key endothelial cell adhesion molecules, expression in mouse aorta. Western blot analysis showed that aortas of SPI − ‐fed mice had reduced expression of CD106 compared to aortas of casein‐fed mice. Further, quantitative RT‐PCR analyses of proximal aorta also showed reduced CD106 mRNA expression in SPI − ‐fed compared to casein‐fed mice. These findings suggest that the atheroprotective effect of isoflavone free soy diet is mediated, in part, by blocking inflammatory responses associated with atherosclerosis. USDA‐CRIS‐6251‐5100002‐06S.