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Absorption kinetics of polyphenols and alteration of antioxidant performance in older adults after ingesting Angelica keiskei
Author(s) -
Switkowski Karen M,
Camacho Camila Renata Correa,
Chen Oliver,
Rasmussen Helen,
Aldini Giancarlo,
Chun HK,
Cho Soomuk,
Park KM,
Russell Robert,
Saltzman Edward,
Blumberg Jeffrey,
Yeum KyungJin
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.321.6
Subject(s) - antioxidant , chemistry , polyphenol , quercetin , high performance liquid chromatography , bioavailability , flavonoid , lutein , food science , carotenoid , medicine , biochemistry , pharmacology , chromatography
Angelica keiskei (AK) is a leafy green vegetable rich in phytochemicals such as carotenoids (C) and flavonoids (F). To determine the absorption kinetics of polyphenols in AK and the alteration of antioxidant performance in vivo, older adults (>60 y) with metabolic syndrome (MetS, n=5) and healthy controls (HC, n=5) consumed 5 g dry AK powder encapsulated in gelatin capsules with a low C & F liquid diet. Plasma samples were collected at baseline, 30 min, 1, 2, 3, 4, 5, 6, 7, and 8 h and analyzed for C and F using HPLC with UV and EC detection, respectively, for Total Antioxidant Performance (TAP) by fluorometry, and for DNA damage using single cell gel electrophoresis (Comet assay). After ingesting AK, increases in plasma quercetin were observed at 1–3 and 6–8 h in the HC and at all time points in the MetS groups compared to baseline (p<0.05). Plasma lutein was elevated in the HC group at all time points and at 6–8 h in the MetS group compared to baseline (p<0.05). Increase in TAP were observed at 1, 3, 6, and 7 h in the HC group and at 1 and 5h in the MetS group compared to baseline (p<0.05). Baseline DNA damage was more than 10% higher in the MetS compared to the HC group (p<0.05). Thus, phytochemicals in AK are bioavailable and can increase antioxidant performance in both HC and MetS subjects. [Supported by USDA ARS #1950‐51000‐065‐08S, USA and BioGreen 21 #20070301034009 RDA, Korea]