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Mechanism of Chromatin Remodeling and Recovery During Passage of RNA Polymerase II
Author(s) -
Studitsky Vasily M,
Kulaeva Olga,
Gaykalova Daria,
Pestov Nikolai,
Golovastov Viktor,
Vassylyev Dmitry,
Artsimovitch Irina
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.310.3
Subject(s) - nucleosome , chromatin , chromatin remodeling , rna polymerase ii , histone , chemistry , microbiology and biotechnology , histone methylation , transcription (linguistics) , biology , dna , promoter , biochemistry , gene , gene expression , dna methylation , linguistics , philosophy
Transcription of eukaryotic genes by RNA polymerase II (Pol II) is typically accompanied by nucleosome survival and minimal exchange of histones H3/H4. The mechanism of survival and recovery of chromatin structure remains obscure. In our recent studies we have shown how transcription through chromatin by Pol II is uniquely coupled with nucleosome survival. Structural modeling and functional analysis of the intermediates of transcription through a nucleosome was conducted. When Pol II approaches the area of strong DNA‐histone interactions, a small intranucleosomal DNA loop (zero‐size or Ø‐loop) containing transcribing enzyme is formed. During formation of the Ø‐loop, the recovery of DNA‐histone interactions behind Pol II is tightly coupled with their disruption ahead of the enzyme. This coupling is a distinct feature of the Pol II‐type mechanism that allows further transcription through the nucleosome, prevents nucleosome translocation and minimizes displacement of H3/H4 histones from DNA during enzyme passage.