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Binge drinking exacerbates asthmatic‐like pulmonary inflammation in cockroach‐allergen (CRA) induced allergic mice
Author(s) -
Bouchard Jacqueline,
Kim Jiyoun,
Vaickus Louis,
Remick Daniel
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.31.2
Subject(s) - eotaxin , bronchoalveolar lavage , medicine , immunoglobulin e , eosinophil , immunology , inflammation , asthma , lung , endocrinology , chemistry , antibody
This study investigates the effects of binge drinking on pulmonary inflammation in CRA‐induced murine asthma. Outbred mice were immunized intratracheally with CRA and challenged 2 times (Day 14 and 21). On Day 21 the Ethanol and Water groups were given 300μl of 32% ethanol or water by oral gavage 30 minutes prior to final challenge (FC). Blood ethanol levels peaked 1 hour post‐gavage (0.13%) and were undetectable by 5 hours. At 1.5 hours post‐FC, acute alcohol consumption led to an increase in bronchoalveolar lavage absolute cells, eosinophils, neutrophils, eotaxin‐1, RANTES, KC, and MIP‐2, and an increase in plasma IgE levels. At 5.5 hours post‐FC AHR exacerbation was measured by increases in enhanced pause (PenH) and time of inspiration (Ti), and decreases in minute ventilation (MV) and peak inspiratory flow (PIF). N = 3–4 mice per group; *pg/ml, **ng/ml; values ± SEM. Supported by 5R01ES013538‐04.Water Ethanol P‐valueAbsolute cells x 10 3 140 ± 21 329 ± 17 <0.001 Eosinophils x 10 3 42 ± 6 160 ± 21 0.001 Neutrophils x 10 3 1.4 ± 0.5 12.3 ± 5.0 0.037 Eotaxin‐1* 12.4 ± 4.5 34.3 ± 4.9 0.009 RANTES* 2.3 ± 1.1 6.3 ± 1.2 0.025 KC* 1810 ± 210 3884 ± 757 0.019 MIP‐2* 353 ± 58 1022 ± 172 0.005 IgE** 20 ± 4 53 ± 12 0.022 PenH (% above base) 38 ± 25 463 ± 246 0.048 Ti (milliseconds) 72 ± 6 93 ± 8 0.042 PIF (ml air/second) 5.1 ± 0.1 3.9 ± 0.4 0.014 MV (ml air/minute) 65 ± 5 46 ± 4 0.018