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How enzymes work
Author(s) -
Herschlag Daniel
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.302.1
Subject(s) - enzyme , function (biology) , work (physics) , chemistry , computational biology , biochemical engineering , biology , biochemistry , physics , engineering , microbiology and biotechnology , thermodynamics
Decades of research in enzymology has remarkably advanced our understanding of enzyme function. Indeed, it is now possible to draw convincing reaction mechanisms for the vast majority of enzymes. As we move to confront challenges in understanding enzyme evolution and the design of enzymes that catalyze novel reactions, a still‐deeper understanding of the structural and energetic underpinnings of catalysis will be required. We have chosen particularly tractable enzymes to ask specific questions about the nature of complementary interactions with transition states and the electrostatic environment within the active site, and we have approached these questions with a battery of structural, biophysical, chemical and biochemical approaches.