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Effects of leucine and calcitriol on monocyte‐vascular endothelial cell adhesion
Author(s) -
Curry Benjamin,
Biggerstaff John,
Zemel Michael B.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.230.5
Subject(s) - calcitriol , monocyte , chemistry , calcium , endocrinology , medicine , cytokine , biology
We previously demonstrated that calcitriol and leucine modulate adipocyte and macrophage cytokine production. Since several of the affected cytokines may modulate vascular infiltration by monocytes, we have tested the hypothesis that calcium (via calcitriol suppression) and leucine attenuate vascular infiltration. Adipocytes were treated with vehicle, calcitriol (to mimic calcium deprivation), leucine or both for 48 hr and conditioned medium (CM) collected and incubated with fluorescently labeled U937 monocytic cells perfused across monolayers of EA hy.926 endothelial cells; adherence was then quantified by microscopy and image analysis. Results are presented as a Monocyte:Endothelial cell ratio (M:E). Calcitriol (10 nM) increased the M:E from 0.088+0.048 to 0.201+0.112 (228% increase, p = 0.0001). Conversely, leucine (0.5 mM) markedly decreased the M:E to 0.013+0.011 (p = 0.001); moreover, addition of leucine CM to the calcitriol CM treatment abolished the effects of calcitriol. A parallel experiment using flow cytometry of CD11b expression on U937 cells under parallel conditions showed an identical trend to the microscopy data. These data show that monocyte adhesion to endothelial cells is decreased in the presence of leucine and increased by calcitriol, suggesting that foods rich in calcium and leucine may be beneficial in reducing monocyte adhesion. Supported by the National Dairy Council.