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Genetic polymorphisms in bovine ferroportin are associated with longissimus dorsi muscle iron content
Author(s) -
Duan Qing,
Reecy James,
Tait Richard,
Liu Qi,
Eenennam Allison,
Mateescu Raluca,
Garmyn Andrea,
Beitz Donald
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.229.2
Subject(s) - single nucleotide polymorphism , genotype , biology , genetics , exon , genetic association , locus (genetics) , purebred , gene , breed
We hypothesized that genetic polymorphisms in ferroportin (Fpn), the only known cellular iron exporter, could influence muscle iron content. The objective of this study was to identify single nucleotide polymorphisms (SNPs) in the exons and flanking regions of the bovine Fpn gene and to evaluate the extent to which they were associated with beef iron content. Longissimus dorsi muscle samples were collected from 1086 head of purebred Angus cattle for iron quantification and DNA extraction. All exons and flanking regions of Fpn were sequenced. Eight novel SNPs were identified, among which NC 007300: g.19208 C>T genotype was associated significantly with iron content ( P < 0.005). Cattle with the g.19208 TT genotype had significantly higher amount of iron compared with those with the g.19208 CC and CT genotypes ( P < 0.005). Sequence analysis showed that this locus might be part of an intron enhancer, potentially influencing pre‐mRNA splicing regulation. Similar significant associations of iron content were observed for three other SNPs. In conclusion, the SNPs identified may be used as DNA markers for muscle iron content. Research support: PFIZER Animal Genetics Division Grant Funding Source: PFIZER Animal Genetics Division