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Hepatic One Carbon Metabolism in Diet‐Induced Obesity
Author(s) -
Glier Melissa B,
Sulistyoningrum Dian C.,
Ghosh Sanjoy,
Devlin Angela M
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.228.3
Subject(s) - medicine , endocrinology , fatty liver , obesity , lipid metabolism , cystathionine beta synthase , metabolism , weaning , liver disease , chemistry , biochemistry , disease , methionine , amino acid
Obesity is a major cause of fatty liver disease. We hypothesize that disturbances in liver one carbon metabolism contribute to the pathology of obesity‐related fatty liver disease. We studied the effect of diet‐induced obesity in mice heterozygous for targeted deletion of cystathionine‐β‐synthase (+/−) and C57BL/6J (+/+) mice fed a high fat (60% energy) diet (HF) or a control diet (C) from weaning for 12 weeks. HF+/− and HF+/+ mice had higher body weight (P<0.001), greater fat mass accumulation (P<0.001), higher liver triglycerides (P<0.05), and lower liver cholesterol ester (P<0.001) than C+/− and C+/+ mice, respectively. Independent of diet, C+/− mice had lower liver S ‐adenosylmethionine (AdoMet) (P<0.05) levels, and higher liver S ‐adenosylhomocysteine (AdoHcy) (P<0.001) levels. Both +/− and +/+ mice fed the HF diet had lower levels of liver AdoHcy (P=0.001) than mice fed the C diet. HF+/− mice had lower liver AdoMet levels (P=0.01) than C+/− mice, however this effect was not observed in +/+ mice. These findings suggest that disturbances in liver one carbon metabolism are associated with hepatic lipid accumulation in mice with diet‐induced obesity.