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Gender and folate intake affect choline dynamics and gene expression in mice consuming isotopically labeled choline.
Author(s) -
Jiang Xinyin,
Chew Tina,
Yan Jian,
Wang Wei,
Carrier Bradley,
Lusa Amanda,
West Allyson,
Malysheva Olga,
Caudill Marie A
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.228.1
Subject(s) - choline , choline acetyltransferase , endocrinology , medicine , phosphatidylcholine , methylenetetrahydrofolate reductase , metabolism , choline chloride , chemistry , biology , biochemistry , acetylcholine , phospholipid , gene , membrane , genotype
This study investigated the effects of folate intake, Mthfr deficiency, and gender on the fate of orally consumed choline and on the transcript levels of choline metabolizing genes. Mthfr +/+ or +/− mice (n=40; 20 nonpregnant female, 20 male; age 10 wk) were randomized to a folate sufficient or folate deficient diet for 8 wk during which modest amounts of labeled choline (d9‐choline; 1.6 mM), added to the drinking water, were consumed. Based on the concentrations of deuterium labeled choline metabolites in liver, gender robustly (P<0.01) affected choline flux. Compared to males, females had higher concentrations of d9‐phosphatidylcholine (PC, 1.5 times), d3‐PC (2 times), d9‐glycerophosphocholine (GPC, 4 times), d9‐betaine (2 times) and d6‐dimethylglycine (2 times). Female mice also expressed more PEMT in liver and kidney, more BHMT in kidney, and less BHMT in liver. Folate deficiency also influenced (P<0.05) choline dynamics in female mice with lower hepatic concentrations of d3‐PC and d9‐GPC. Increased expression (~3 times) of hepatic choline dehydrogenase and brain choline acetyltransferase was also observed in both genders. In contrast, Mthfr deficiency had subtle effects on choline metabolism; none of which achieved statistical significance. Taken together, these data show enhanced choline cycling and greater sensitivity to folate intake in nonpregnant female than male mice. Grant Funding Source: Funded in part by NIH Grant No. S06GM053933 and the California Agricultural Research Initiative