Premium
De novo lipogenesis in Type 1 diabetes pre‐ and post‐islet transplant
Author(s) -
Lambert Jennifer Elizabeth,
Ryan Edmond A.,
Thomson Alan B.R.,
Clandinin Michael T.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.210.3
Subject(s) - lipogenesis , medicine , endocrinology , triglyceride , type 1 diabetes , chemistry , islet , cholesterol , immunosuppression , metabolism , diabetes mellitus
Type 1 diabetes (T1D) patients have elevated cardiovascular disease (CVD) risk yet normal blood lipids, suggesting altered lipid metabolism. Islet transplant (ITx) may cure T1D in some people, but immunosuppressive drugs may be hyperlipidemic. The objective was to investigate lipogenesis in T1D and ITx patients using stable isotopes. People with T1D (T1D; 4F/3M), post‐ITx patients (ITx; 3F/5M) and healthy subjects (Control; 4F/2M) participated. Fasting blood samples (0h and 24h) were analyzed for triglyceride (TG). Deuterium‐labeled water was given and stable isotope analysis performed on VLDL TG estimated hepatic synthesis of total and individual fatty acids (FA). Plasma TG was not different between Control (0.84±0.24), T1D (0.95±0.22), or ITx (0.75±0.31). Compared to Control, T1D showed a trend for greater total FA synthesis, of particularly 14:0, 16:0, and 18:0. ITx had reduced total FA synthesis (p<0.01), particularly 14:0, 16:0, 18:0, and 18:1 (p<0.01 for all) compared to T1D. Total FA synthesis was significantly related to plasma TG in ITx only (p<0.05). Synthesis of 16:0 was correlated with plasma TG level in Control and T1D (p<0.05) but not ITx. T1D may have greater hepatic FA synthesis, contributing to CVD risk. In this ITx group, immunosuppression did not raise plasma TG levels and did not have deleterious effects on hepatic FA synthesis. Grant Funding Source : Canadian Institutes of Health Research