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Augmented insulin effects on plasma glucose by cranberry procyanidins in streptozotocin‐induced diabetic rats
Author(s) -
Prior Ronald L.,
Wilkes Sam,
Rogers Theodore J.,
Khanal Ramesh,
Howard Luke
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.209.2
Subject(s) - insulin , streptozotocin , medicine , endocrinology , diabetes mellitus , chemistry , intraperitoneal injection , oral administration
Objectives of this study were to determine if cranberry proanthocyanidins (CPACs) had an antihyperglycemic effect in the presence or absence of insulin in male diabetic Sprague‐Dawley rats. Rats (~250 g)(n=6–10/trt) were given a single intraperitoneal (ip) injection of freshly prepared streptozotocin (STZ) at a dose of 70 mg/kg in 50 mM citrate buffer (pH 4.5). Diabetic animals with hyperglycemia greater than 400 mg/dL on day 7 after STZ injection were used. Treatments included: 1) Oral gavage of tap water; 2) Oral gavage of CPACs in aqueous solution (250 mg/kg body weight); 3) Oral gavage of tap water plus insulin − 9 nmole/animal ip; 4) Oral gavage of CPACs (250 mg/kg body weight) plus insulin − 1.26 nmole/animal ip; 5) Insulin given ip at a dose of 1.26 nmole/animal. Blood samples were collected by tail bleeding, and glucose measured at 0, 30, 60, 90, 120, 150, 210 and 270 min. CPACs did not alter glucose levels compared to water. CPACs plus the low dose of insulin potentiated the insulin effect in lowering glucose equivalent to the high dose of insulin. Area under the curve for blood glucose change following treatment was −22486±6426; − 21219±7322; − 99283±10262; − 89991±4604; − 65605±11463 for treatments 1, 2, 3, 4, and 5 respectively. Cranberry derived procyanidins had an additive antihyperglycemic effect in the presence of a low insulin dose, but not in the absence of insulin. Funded by USDA, ARS and Decas Botanicals.