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Non‐invasive stool‐based detection of newborn infant gastrointestinal development using gene expression profiles derived from exfoliated epithelial cells
Author(s) -
Donovan Sharon Marie,
Davidson Laure A,
Zhao Chen,
Ivanov Ivan,
Goldsby Jennifer S,
Lupton Joanne R,
Mathai Rose Ann,
Monaco Marcia H,
Rai Deshanie,
Russell Michael,
Dougherty Edward R,
Chapkin Robert S
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.206.6
Subject(s) - biology , gene , wnt signaling pathway , gene expression , transcription factor , messenger rna , regulation of gene expression , microbiology and biotechnology , genetics
Understanding of the molecular regulation of intestinal development by human milk has been limited by the lack of non‐invasive approaches. Herein, a novel molecular methodology utilizing stool samples containing intact sloughed epithelial cells was used to identify gene sets (combinations) that were differentially regulated in response to infant feeding. Fecal mRNA was isolated from 3‐month‐old exclusively breast (BF; n=12) and formula (FF; n=10) fed infants. Linear discriminant analysis was used to identify the best single genes and two‐ to three‐gene combinations for distinguishing the feeding groups. EPAS‐1, a transcription factor involved in inducing oxygen‐regulated genes, was identified as a putative “master” regulatory gene using coefficient of determination analysis. Bioinformatic analysis identified differentially regulated processes associated with apoptosis, chemotaxis and development (Wnt and NOTCH pathways). Thus, epithelial cell mRNA isolated from stool has value in terms of characterizing the mechanisms underlying developmentally‐regulated transcriptional activation/repression of genes known to modulate gastrointestinal development. (Funded by Mead Johnson and NIH CA59034, CA129444 , DK71707 and P30ES09106)