Premium
Signal transduction by lipid‐anchored molecules as revealed by single‐molecule tracking
Author(s) -
Kusumi Akihiro
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.188.4
Subject(s) - lipid raft , lyn , microbiology and biotechnology , signal transduction , cell signaling , intracellular , chemistry , cd59 , phosphorylation , raft , biology , complement system , proto oncogene tyrosine protein kinase src , immune system , immunology , polymer , organic chemistry , copolymer
Using simultaneous single‐molecule tracking of CD59, glycosylphosphatidylinositol (GPI)‐anchored receptor for controlling self‐attack of the complement complexes, and intracellular lipid‐anchored signaling molecules, Galphai2, Lyn, and PLCgamma, we were able to observe that these intracellular signaling molecules are transiently recruited to ligand‐induced CD59‐cluster rafts, leading to intracellular IP3‐calcium signaling. The key process was the ligand‐induced clustering of CD59, which in turn induces raft‐like nanodomains by concentrating cholesterol and glycosphingolipids. Such an induced raft then recruits Galphai2 and Lyn via both protein‐protein and lipid‐lipid interactions, leading to their greatly enhanced interactions. Such transient recruitment of signaling molecules appeared in various signaling systems we have observed thus far, and could be a general nature of the cellular signaling.