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Myocardial BMP Can Promote Orientated Protrusion of the Proepicardium Necessary for Entry of Coronary Vessel Precursors and Epicardial Progenitors to the Heart
Author(s) -
Garriock Robert John,
Ishii Yasuo,
Navetta Alicia,
Mikawa Takashi
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.180.2
Subject(s) - noggin , heart development , cardiology , vasculogenesis , medicine , anatomy , chemistry , microbiology and biotechnology , progenitor cell , bone morphogenetic protein , biology , stem cell , embryonic stem cell , biochemistry , gene
The coronary vessel precursors and epicardium derive from a separate tissue rudiment outside of the heart called the proepicardium (PE). The PE protrudes to the heart and makes contact with the atrioventricular junction/inner curvature (AV/IC) region despite being closer to the sinoatrium. The attachment of the PE to the heart is an essential step in coronary vasculogenesis and formation of the epicardium. However, it is unknown how the specificity of PE attachment to the AV/IC is achieved. We demonstrate that BMP‐signaling from the myocardium specifies the attachment site of the PE to the heart. We show that Bmp2, which is expressed specifically at the AV/IC region of the heart, can signal alternative sites of PE attachment when misexpressed outside the AV/IC region. In contrast, misexpression of a BMP‐antagonist, Noggin, blocks PE attachment to the heart. Finally, PE explants preferentially protrude toward the AV/IC and this preference can be mimicked by BMP2/4. These results support a model in which BMP signals from the myocardium regulate PE protrusion and contact with a specific site on the heart. Grant Funding Source : NIH