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Immunolocalization of VEGF and VEGFR 1 & 2 in embryonic lung tissues
Author(s) -
McNeil Rosie Thecla,
Maina John N,
Kramer Beverly
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.172.2
Subject(s) - lung , vascular endothelial growth factor , immunohistochemistry , paraformaldehyde , embryonic stem cell , biology , staining , pathology , vegf receptors , chemistry , medicine , immunology , cancer research , biochemistry , gene
Eb 2010 Abstract The role of vascular endothelial growth factor (VEGF) and its receptors 1 and 2 (flt and flk) have been identified in mammalian lung development but there are few studies concerning their role in avian lung. The objective of this study was to determine whether VEGF, flt and flk are expressed in the developing avian lung between stages 20 to 40. Sections, 4 micrometers thick from 4% paraformaldehyde‐fixed, paraffin‐embedded embryonic lung buds and were immunolocalized for the growth factors using standard avidin‐biotin peroxidase immunohistochemical techniques. Mouse kidney and lung were used as positive control tissues. Positive staining for VEGF presented as diffuse cytoplasmic staining which was first observed at stage 27 with the intensity increasing towards stage 40. Staining for flt and flk were however less intense than that of VEGF. We concluded that VEGF, VEGFR1 and VEGFR2 may play significant roles in avian lung development. Grant Funding Source : Faculty Research Grant