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Evaluation of MRSA Detection By Genetic v/s Chromogenic v/s Conventional Methods in New Admission to SICU/TICU/NICU Units at Hamad Medical Corporation
Author(s) -
Doiphode Sanjay,
Thani Asma Al,
AlRemaihi Hanan,
Yagoob Aisha,
AlMoklf Fatema
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.111.12
Subject(s) - chromogenic , medicine , sccmec , staphylococcus aureus , genexpert mtb/rif , population , methicillin resistant staphylococcus aureus , biology , pathology , environmental health , tuberculosis , art , mycobacterium tuberculosis , bacteria , visual arts , genetics
Methicillin‐resistant Staphylococcus aureus (MRSA) is usually acquired during exposure to hospitals and other healthcare facilities, and causes a variety of serious health care associated infections. The aim of this study is to evaluate, compare and detect MRSA using genetic, chromogenic and conventional methods in admission to SICU/TICU/NICU at Hamad Medical Corporation. A total of 30 specimens were collected from patients admitted to SICU/TICU/NICU and each specimen was performed in 4 different methods including 1‐ conventional method using: Mannitol salt agar (MSA), 2‐ chromogenic using CHROMagar and 2 genetic methods; 3‐Smart Cycler BD GeneOhm™ MRSA and 4‐ GeneXpert® MRSA Assay. The results indicated that MRSA was detected in 10% of the study population. The distribution of positive samples using MSA Vs CHROMagar Vs Gene OHM Vs GenXpert methods was as following: 28%, 18%, 27% and 27% respectively. So we can conclude that traditional methods for screening of MRSA with the conventional and chromogenic methods are still useful to determine the type of MRSA and both methods are complementary to each others and introducing the genetic methods had a great advantage in solving problems facing the hospital with turnaround time.