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Restoration of fatty acid but not glucose utilization in the infarcted heart following stem cell therapy
Author(s) -
Hughey Curtis,
Ma Lianli,
James Freyja,
Young Pampee Paul,
Wasserman David,
Rottman Jeff,
Shearer Jane
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.110.12
Subject(s) - ventricle , medicine , stem cell therapy , myocardial infarction , stem cell , fatty acid , cardiology , endocrinology , biochemistry , chemistry , transplantation , biology , genetics
Aim To determine whether stem cell therapy reduces abnormal cardiac substrate utilization following a myocardial infarction (MI). Methods Three treatment groups, SHAM, MI and MI‐STEM receiving intramyocardial injection of mesenchymal stem cells. Echocardiography was performed at 0, 1 and 3wk. C57BL/6J mice underwent a hyperinsulinemic‐euglycemic clamp 4wk following treatment (n=8–11). To assess indices of whole body and cardiac long chain fatty acid (LFCA) and glucose utilization (R(f), R(g); umol/g wet weight/min), 2‐deoxy[(3)H]glucose and [125I]‐15‐(rho‐iodophenyl)‐3‐R,S‐methylpentadecanoic acid were administered. Both the peri‐infarct (PI) region and a non‐involved (NI) area of the left ventricle were examined. Results No differences in whole body substrate utilization or insulin sensitivity, as assessed by glucose infusion rate were apparent between treatments. In the PI region, MI resulted in declines in R(f) compared to SHAM (0.25 vs 0.44). This decline was mitigated in MI‐STEM (0.33 vs 0.44). In contrast, R(g) was diminished in both the MI and MI‐STEM groups in the PI (1.77 vs 2.0 vs 3.87) compared to the SHAM. Additionally, R(f) and R(g) in the NI was not different between groups. Conclusions Stem cell therapy assists in reducing the metabolic abnormalities following a MI. Specifically, it minimizes MI‐induced declines in R(f) but not R(g).