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Rapid and Direct Quantitation of Gas Content in Liquids, Emulsions, and Blood
Author(s) -
Rodriguez Juan,
Newell Clayton,
Simpkins Cuthbert
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1065.21
Subject(s) - mass spectrometry , chromatography , chemistry , analytical chemistry (journal) , whole blood , gas phase , gas chromatography , content (measure theory) , co2 content , carbon dioxide , organic chemistry , mathematical analysis , mathematics , immunology , biology
Here we describe new methodology for the rapid measurement of gas content in fluids, including simple liquids, emulsions, and blood. Unlike blood gas analyzers, our experimental approach measures gas content directly, i.e. without inferring it from the partial pressure of the gases in the aqueous phase. Our method incorporates a mass spectrometer detector that provides molecular specificity for O2, CO2, or other gases, so they may be detected by themselves or simultaneously. Mass spectrometry also affords ultra‐sensitive detection, which enabled us to measure O2 and CO2 content from 20 μL of whole blood with signal to noise ratios exceeding 1000:1. The ability to quantify gas content in microliter samples should enable the use of small animals for studying gas transport in plasma, whole blood, or even blood mixed with blood substitutes.