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Immunoparalysis: a new approach for its early detection and resolution
Author(s) -
Torbati Dan,
Lin WeiChiang,
Raszynski Andre,
Totapally Balagangadhar R.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1065.17
Subject(s) - medicine , immunology
Persistence of the anti‐inflammatory reactions leads to immunoparalysis, characterized by reduced expression of pro‐inflammatory cytokines and the human leukocyte antigen‐DR in the blood. Reversal of immunoparalysis, in critically ill patients, could be life saving. Currently, cytofluorometric methods are used to detect changes in leukocytes functions, while the enzyme‐linked immunosorbent assay is used to measure the concentration of pro‐ and anti‐inflammatory cytokines. We propose to quantify the cumulative impact of the multiple inflammatory mediators, which are involved in development of immunoparalysis, by quantitative real time polymerase chain reaction. A simultaneous profiling of the pro‐ and anti‐inflammatory mediators at the mRNA level, combined with appropriate statistical analyses, may result in early detection of immunoparalysis. Statistically, a non‐parametric ANOVA could be used to compare expression scores of multiple mediators based on “fold change”, or the ratios of pro‐ and anti‐inflammatory mediators. Such ratios could be also evaluated by simple or complex chi square test if categorized as either opposing, or promoting immunoparalysis. The statistical outcomes could be used as indices for the onset/resolution of immunoparalysis in individuals, or a cohort of patients in clinical trials. The sensitivity and specificity of this approach warrants investigation in septic, and/or trauma patients.