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Effects of chronic antioxidant treatment on AT‐1 receptor, NAD(P)H oxidase subunits and main antioxidant enzymes gene expression in the kidneys of 2K‐1C rats
Author(s) -
Nishi Erika Emy,
OliveiraSales Elizabeth B,
Bergamaschi Cassia T,
Oliveira Thais G. C.,
Boim Mirian A,
Campos Ruy R
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1059.11
Subject(s) - oxidative stress , endocrinology , medicine , antioxidant , nad(p)h oxidase , glutathione peroxidase , chemistry , gene expression , kidney , oxidase test , catalase , nadph oxidase , enzyme , biochemistry , gene
Previous studies showed that chronic treatment with an antioxidant: vitamin C (vC) improves hypertension and sympathetic vasomotor hyperactivity in an Ang II‐dependent model of renovascular arterial hypertension. To test the hypothesis that oxidative stress is associated with NAD(P)H oxidase activation by Ang II, gene expressions of AT‐1 receptor (AT 1 R), NAD(P)H oxidase subunits (p47phox and gp91phox) and main antioxidant enzymes were evaluated in the renal cortex of 2 kidney‐1 clip model (2K1C, 6 weeks after clipping) rats before and after vC (150mg/kg/day). Gene expression of AT 1 R was elevated (51%) in the clipped kidney (CK) and reduced (47%) in the nonclipped kidney (NK) of 2K1C group. vC reduced AT 1 R expression only in the CK (31%). Elevated gene expression of p47phox and gp91phox was observed only in the CK of 2K‐1C group (184% and 132%, respectively) and vC produced no changes in these expressions. Gene expression of catalase was reduced in the CK (70%) and NK (83%) of 2K1C rats. vC increased gene expression of glutathione peroxidase (GPx) in the CK (185%) and NK (212%) of 2K1C. The present study suggests that renal oxidative stress may be related to elevated AT 1 R and NAD(P)H oxidase subunits gene expression in the CK of 2K1C rats and vitC treatment may produce beneficial effects by AT 1 R downregulation in the CK and increase in GPx gene expression in the kidneys of 2K1C rats. Supported by FAPESP (07/56925‐1)