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Transplantation of mesenchymal stem cells fails to provide a synergistic effect on functional recovery of transected nerve with exercise‐treated rats
Author(s) -
Yang ChenChia,
Hsieh YuehLing,
Ou HsiuChung
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1058.6
Subject(s) - transplantation , medicine , mesenchymal stem cell , lesion , sciatic nerve , electrophysiology , ankle , surgery , anatomy , anesthesia , pathology
This study examined whether transplantation of mesenchymal stem cells (MSC) in combination with exercise had synergistic effects leading to functional recovery that is greater than exercise alone. Adult male SD rats received a sciatic nerve transection were divided into four groups: denervated (control), denervated + exercise (control + Ex), denervated + MSC transplantation (MSC), and denervated + MSC transplantation + exercise (MSC + Ex). MSC was injected into the lesion site in MSC‐treated groups and culture medium was injected as control. Twelve hours after surgery, two exercise groups swam for 7 days. Functional assessments including sciatic function index (SFI), vertical activity (VA) of locomotion, angle of ankle (AA), and electrophysiological studies were performed for monitoring the recovery of motor function. No significant differences in SFI, VA and AA were found between MSC + Ex and control + Ex groups. Furthermore, there were no significant differences in amplitudes and onset latencies of compound muscle action potentials between two groups. Morphological study revealed prominent axonal degeneration in lesion nerves. Results showed no noticeably synergistic effect of MSC transplantation on functional recovery of transected nerve treated with swimming exercise. This study was supported by a grant from the National Science Council (95‐2314‐B‐241‐003) of Taiwan.