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Rapid Cold Hardening in Drosophila requires MAPK signaling
Author(s) -
Kelty Jonathan,
Ancevski Aleksandar
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1055.18
Subject(s) - drosophila melanogaster , mapk/erk pathway , melanogaster , microbiology and biotechnology , biology , cryoprotectant , signal transduction , protein kinase a , kinase , biochemistry , gene , cryopreservation , embryo
Increasing evidence supports the hypothesis that rapid cold hardening (RCH) protects insects in nature. However, the cellular/biochemical mechanisms of RCH remain unclear. In Sarcophaga crassipalpis RCH involves accumulation of glycerol and Mitogen Activated Protein Kinase (MAPK) signaling pathways. For Drosophila melanogaster RCH correlates with changes in membrane lipid composition, but does not appear to involve the production of low‐molecular weight cryoprotectants. The present study utilized a pharmacological approach to determine whether RCH in D. melanogaster involves MAPK signaling. Rearing D. melanogaster on yeast paste containing the MAPK inhibitor SB203580 for 2 days following eclosion impaired their ability to rapidly cold harden (evaluated as resistance to mortality caused by cold shock injury), in a dose dependent manner. These data suggest that MAPK signaling is important to RCH in D. melanogaster .