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Effects of chronic iron deficiency anemia on the protein expression of rat thalamus
Author(s) -
Kawano Fuminori,
Oke Yoshihiko,
Nakai Naoya,
Ohira Takashi,
Nomura Sachiko,
Fujita Ryo,
Ohira Yoshinobu
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1054.5
Subject(s) - thalamus , western blot , medicine , iron deficiency , iron deficiency anemia , endocrinology , anemia , hematocrit , hemoglobin , protein expression , chemistry , biochemistry , gene , radiology
The present study was carried out to investigate the effects of anemia on the protein expression in rat thalamus. The powdered diet containing 100 or 3 ppm iron was fed in rats with the age of 3‐week. After 20 weeks of supplementation, blood and thalamus including hypothalamus were sampled from both groups. The hematocrit and hemoglobin content were 44 and 15 g/dl in the 100 ppm group, respectively. However, they were less (17 and 4 g/dl) in the 3 ppm group. The two‐dimensional electrophoresis was performed using the homogenate of thalamus. As the result, 73 kDa heat shock protein (HSP73) was found in the spot which expression level was greater in 3 ppm group. Further, western blot was performed to check the quantitative expression level. Although the level of total HSP73 was greater (+89%) in the 3 ppm group, no differences were seen if they were compared among spots with the different isoelectric points. The iron deficiency‐induced anemia may cause some changes in thalamus function via HSP73 expression. This study was supported by Grant‐in‐Aid for Scientific Research S (19100009) and Exploratory Research (20650104) from JSPS, and Young Scientists B (21700656) from MEXT.