Premium
TRPV1‐MEDIATED MODULATION OF SYNAPTIC INPUTS IN IDENTIFIED KIDNEY‐RELATED PRE‐SYMPATHETIC IML NEURONS
Author(s) -
Derbenev Andrei V,
Duale Hanad
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1051.9
Subject(s) - trpv1 , neuroscience , patch clamp , electrophysiology , chemistry , transient receptor potential channel , medicine , endocrinology , receptor , biology
Synaptic balance in the neurons of the intermediolateral cell column (IML) is a critical component in the central pathways mediating sympathetic innervation of the kidney. Transient receptor potential vanilloid type 1 (TRPV1) have potent effects on autonomic motor neurons and are expressed in the IML, but effects of TRPV1 activation in the IML is not known. Whole‐cell patch‐clamp recordings in longitudinal spinal cord slices were used to characterize synaptic activity of kidney‐related pre‐sympathetic IML neurons identified with the transynaptic retrograde viral vector PRV‐152, to test the hypothesis that activation of TRPV1 enhances synaptic activity to these neurons. The resting membrane potential was −51±4 mV and input resistance was 448±39 MΩ. At a holding potential of −60 mV, we observed spontaneous EPSCs at a frequency of 3.1±1 Hz with an average amplitude of 11.5±2 pA. At a holding potential of −10 mV, we observed spontaneous IPSCs with a frequency of 1.4±0.3 Hz and an amplitude of 19±9 pA. Immunohistochemical studies showed that TRPV1 were distributed throughout the IML and co‐localized with kidney‐related IML neurons. Application of the TRPV1 agonist capsaicin increased the frequency of spontaneous IPSCs and EPSCs in kidney‐related pre‐sympathetic IML neurons by 150%. This mechanism can modulate blood pressure and, by inference, hypertension. Supported by NIH HL091293‐01A1; 3R21HL091293‐01A1S1.