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Hyperinsulinemia and ectopic fat deposition develop in the face of hyperadiponectinemia in young obese rats
Author(s) -
Ronis Martin J,
Marecki John C,
Shankar Kartik,
Badger Thomas M
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.105.6
Subject(s) - medicine , endocrinology , hyperinsulinemia , adiponectin , insulin resistance , fatty liver , insulin , chemistry , disease
The role of reduced adiponenctin signaling in childhood obesity is unclear. Weanling male Sprague‐Dawley rats were overfed a high fat diet via total enteral nutrition. Excessive caloric intake led to increased weight and fat mass; dyslipidemia; ectopic fat deposition; and hyperinsulinemia (P<0.05). Expression of fatty acid transporter CD36 was elevated in both liver and muscle (P<0.05). Akt phosphorylation was elevated in liver but not muscle and hepatic nuclear FoxO1 protein reduced (P<0.05). Overfeeding increased serum adiponectin from 24.6±1.9 μg/ml to 46.3±5.9 μg/ml (P<0.05) correlating with increased fat mass. TNF‐α expression in fat was unchanged. AMP kinase phosphorylation, PPAR‐α expression and fatty acid oxidation was elevated in both liver and muscle (P<0.05). Thus excessive intake of high fat diet in young rats resulted in “adiponectin‐independent” increases in serum insulin and elevated ectopic fat deposition. Fatty acid transport appears to be the major mechanism underlying ectopic fat deposition. These data suggest age‐related differences in the role of adiponectin in pathological responses associated with obesity. USDA CRIS‐6251‐51000‐007‐03S.