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Protein S‐nitrosylation prevented by resistive vibration exercise (RVE) in human skeletal muscle following 60‐days of bed rest immobilization (BBR2‐2 Study)
Author(s) -
Salanova Michele,
Schiffl Gudrun,
Gutsmann Martina,
Felsenberg Dieter,
Belavý Daniel,
Blottner Dieter
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1046.17
Subject(s) - bed rest , muscle atrophy , skeletal muscle , medicine , atrophy , isometric exercise , muscle biopsy , endocrinology , chemistry , cardiology , biopsy
Reactive Nitrogen Species (RNS) are important regulators of signalling pathways in muscle atrophy during prolonged disuse. We used the bed rest analogue (2 nd Berlin Bed Rest Study, BBR2‐2) as a model of muscle inactivity, to study the pattern of S‐nitrosylated proteins in healthy human subjects following prolonged immobilization with and without exercise countermeasures. Twenty‐four healthy male subjects underwent 60‐days of 6° head down tilt bed‐rest (BR) and were randomized to an inactive control group (BR‐CTR, n=8), a high‐load resistive exercise group (BR‐RE, n=8) and a high‐load resistive vibration exercise (BR‐RVE, n=8) group. Muscle biopsy from m. soleus (SOL) and m. vastus lateralis (VL) were performed before and after BR and the pattern of S‐nitrosylated proteins were investigated by the “biotin switch assay”. Preliminary results showed that the level of S‐nitrosylated muscular proteins was altered after BR in all groups (i.e., BR‐CTR group: +10% SOL; +15% VL; BR‐RE group: no change in SOL, +18% in VL; BR‐RVE group: −19% in SOL, −15% in VL). More detailed proteome analysis of s‐nitrosylated proteins is presently underway. Vibration exercise thus serves as an effective exercise countermeasure protocol to prevent oxidative stress during disuse‐induced skeletal muscle atrophy or exposure to microgravity. Sponsor: Deutsches Zentrum für Luft‐und Raumfahrt (DLR), Germany (# 50WB0821 to D.B.)