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Progesterone administration antagonizes the effect of estradiol on endothelium‐dependent vasodilation in young healthy women
Author(s) -
Miner Jennifer Ann,
Smith Michael M,
Martini Emily R,
Brunt Vienna E,
Kaplan Paul F,
Halliwill John R,
Minson Christopher T
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1041.22
Subject(s) - medicine , endocrinology , estrogen , vasodilation , brachial artery , hormone , endothelium , antagonist , nitric oxide , receptor , blood pressure
Purpose To examine the acute effects of progesterone and estrogen on endothelium‐dependent vasodilation in healthy reproductive‐aged women. Methods We suppressed endogenous estrogens and progesterone in 16 premenopausal women for 10 days using a gonadotropin‐releasing hormone antagonist (GnRHa). On day 4 , subjects were tested and then supplemented either 0.1 mg estradiol (GnRHA+E 2 ; N=8) transdermally or 200 mg progesterone (GnRHa+P 4 ; N=8) orally per day. On day 7 subjects were tested and began supplementation with both hormones (GnRHa+P 4 +E 2 ), and were tested again on day 10 . Flow‐mediated vasodilation (FMD) of the brachial artery was assessed using B‐mode arterial ultrasound, combined with synchronized Doppler analysis. Results Significant differences were observed when comparing FMD in GnRHa (7.85 ± 2.77%) and GnRHa+E 2 conditions (10.14 ± 1.40%; p<0.05). The E 2 increase was abolished when progesterone was also supplemented (6.27 ± 2.41%). In contrast, GnRHa+P 4 (6.66 ± 2.56%) did not significantly alter FMD from GnRHa (7.80 ± 2.76%) and GnRHa+P 4 +E 2 (7.40 ± 3.02%). However, a multi‐level prediction model demonstrates progesterone (p<0.02) and estradiol (p<0.01) predict FMD within subjects. Conclusion These data suggest that acute progesterone administration antagonizes the effect of estrogen on endothelium‐dependent vasodilation. Supported by NIH Grant HL081671.