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Chronic angiotensin II (Ang II) increases renal oxidative stress in male spontanously hypertensive rats (SHR), but not in female SHR
Author(s) -
Brinson Krystal N.,
Sullivan Jennifer C.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1041.1
Subject(s) - oxidative stress , medicine , nox4 , endocrinology , catalase , nadph oxidase , angiotensin ii , chemistry , blood pressure
Male SHR are more sensitive to Ang II‐induced increases in blood pressure than females. Ang II‐induced oxidative stress contributes to hypertension in males; less is known regarding Ang II‐induced oxidative stress in females. We hypothesize female SHR have less Ang II‐induced oxidative stress compared to males. 13 week old control and Ang II infused (200 ng/kg/min, 14 day minipump) male and female SHR were studied (n=3–4). The renal cortex was isolated to measure O 2 ·− levels via lucigenin. Males had higher O 2 ·− levels than females (see figure, * indicates p<0.05). Ang II increased O 2 ·− levels in males(+ indicates p<0.05) yet levels were similar in females and female + Ang. Renal cortical NADPH oxidase NOX4 and p22 phox subunits, SOD and catalase protein expression were assessed. p22 phox expression was comparable in males (4.0±0.2 rdu) and male + Ang (4.0±0.2 rdu), however, Ang II tended to decrease p22 phox in females (female: 4.0±0.3 rdu; female + Ang: 3.4±0.1 rdu, p=0.07). Ang II decreased ECSOD expression in males (male: 24±1; male + Ang: 20±1 rdu, p<0.05), with no effect in females (female: 20±2; female + Ang: 22±1 rdu). NOX4, CuZn SOD, MnSOD, and catalase expression were comparable among groups. Ang II infusion increased oxidative stress in male, but not female SHR. We hypothesize that this is related to increased antioxidant potential and lowered NADPH oxidase activity in female SHR with Ang II infusion. Funded by NIH.