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Rhythmic mechanical deformation of the human forearm increases venous plasma [ATP] and ATP release
Author(s) -
Crecelius Anne R.,
Kirby Brett S.,
Richards Jennifer C.,
Voyles Wyatt F.,
Dinenno Frank A.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1039.10
Subject(s) - forearm , adenosine triphosphate , vasodilation , medicine , chemistry , endocrinology , blood flow , biophysics , anatomy , biology
Accumulating evidence indicates that circulating adenosine triphosphate (ATP) evokes vasodilation and assists in skeletal muscle blood flow regulation during exercise. Mechanical deformation of erythrocytes can stimulate ATP release in vitro, and we have demonstrated that rhythmic mechanical deformation of the forearm increases blood flow (FBF) via vasodilation. Thus, we tested the hypothesis that mechanical effects of muscle contractions increase [ATP] v and ATP release in vivo independent of changes in tissue metabolic demand. In 6 young adults, blood samples were obtained from a deep venous catheter and were mixed in stop solution to preserve [ATP] v for measurement. We measured FBF (Doppler ultrasound) and plasma [ATP] v and calculated forearm ATP release (FBF x [ATP] v ) during rhythmic forearm compressions via a blood pressure cuff at 3 graded extravascular pressures (50, 100 and 200 mmHg). [ATP] v was significantly increased at each pressure compared with rest (range: 581–609 vs 237 ± 9 nmol/l; P < 0.05). Additionally, forearm compressions evoked a graded increase in FBF and thus ATP release was graded with extravascular pressure ( P < 0.05). We conclude that rhythmic mechanical deformation of the forearm increases [ATP] v and ATP release, and that this mechanical effect of muscle contractions could contribute to the observed increases in [ATP] v and ATP release during exercise. Supported by HL‐087952