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Baicalein attenuates oxLDL‐induced oxidative functional damages in endothelial cells
Author(s) -
Lin ChunChao,
Ou HsiuChung,
Pi I,
You YaChing
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1038.2
Subject(s) - baicalein , scutellaria baicalensis , chemistry , oxidative stress , baicalin , apoptosis , umbilical vein , pharmacology , reactive oxygen species , inflammation , microbiology and biotechnology , biochemistry , immunology , medicine , in vitro , biology , traditional chinese medicine , pathology , alternative medicine , high performance liquid chromatography , chromatography
Endothelial apoptosis is a driving force in atherosclerosis development. Oxidized low‐density lipoprotien (oxLDL) pormotes inflammatory and thrombotic processes. Baicalein, a flavonoid obtained from the root of Chinese medicinal herb Scutellaria baicalensis, possesses a multitude of pharmacological activities including anti‐oxidant, anti‐inflammation and anti‐tumor. In this present study, we tested the hypothesis that baicalein could protects vascular endothelial cells against the proatherosclerotic stressor oxLDL and explored the possible mechanisms. Human umbilical vein endothelial cells (HUVECs) were incubated with baicalein (2.5–20 μM) for 2 hours and then incubated with oxLDL (150 μg/ml) for an additional 24 hours. Our results showed that baicalein ameliorated the oxLDL‐diminished eNOS protein expression and reduced the oxLDL‐induced adhesion molecules and the adherence of monocytic THP‐1 cells to HUVECs. Furthermore, several apoptotic features caused by oxLDL including ROS production, intracellular calcium accumulation, mitochondrial membrane potential collapse, disturbed the balance of bcl‐2 family proteins, and triggered subsequent cytochome c release to cytosol and activation of caspase‐3 were attenauted by pretreatment with baicalein. Findings from this study suggest that baicalein may have clinical implications in the prevention of atherosclerotic vascular disease.