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Targeted VEGF Therapy Favorably Alters Collagen Deposition and Quality after Myocardial Infarction
Author(s) -
Cheheltani Rabee,
Rosano Jenna M,
Wang Bin,
Pleshko Nancy,
Kiani Mohammad F
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1031.7
Subject(s) - medicine , myocardial infarction , heart failure , masson's trichrome stain , trichrome , cardiology , vascular endothelial growth factor , ventricular remodeling , cardiac function curve , perfusion , fibrosis , pathology , h&e stain , immunohistochemistry , vegf receptors
Every year 1.5 million people in the United States alone suffer from myocardial infarction (MI). MI may cause adverse cardiac remodeling, leading to congestive heart failure. Previously, we have shown that a low dose of vascular endothelial growth factor (VEGF) targeted via anti‐P‐selectin immunoliposomes to the infarcted heart improves cardiac function and vascular perfusion. The aim of this study is to examine the role of targeted VEGF in cardiac remodeling after MI. An MI was induced in rats, and treated with anti‐P‐selectin conjugated immunoliposomes containing VEGF (0.12 ug/kg), or left untreated. After four weeks, hearts were excised and stained with Gomori's trichrome and Picrosirius red for collagen. Fourier transform infrared imaging spectroscopy (FT‐IRIS) data was obtained from sections of the left ventricular wall to assess molecular changes. In hearts treated with targeted VEGF therapy, collagen deposition was reduced as evidenced by collagen volume fraction in the anterior wall (50.0 ± 4.5 % vs. 86.7 ± 5.9 %, untreated), and the percentage of circumference that is scar tissue (23.9 ± 5.9 % vs. 39.2 ±1.8 %). The FT‐IRIS data demonstrated that the ratio of mature to immature crosslinks was greater in the treated tissue. Together, these data show that targeted VEGF therapy modifies both collagen quality and quantity, suggesting that it favorably alters cardiac remodeling. Supported by a grant from the AHA.