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Transforming Growth Factor‐beta Expression in Obesity‐Linked kidney Disease
Author(s) -
Maddox David A.,
Ingebrigston Dane
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1030.6
Subject(s) - medicine , endocrinology , transforming growth factor , obesity , gene expression , kidney , kidney disease , biology , gene , biochemistry
Objective To gain insight into the matrix synthesis cascade in obesity‐linked renal disease. Methods The study used obese and lean Zucker rats. Obese rats six weeks of age were fed either ad libitum (Ob‐ ad lib) or restricted (Ob‐Res) in food intake to that consumed by lean Zucker controls, then tested at 6, 12, and 18 weeks for each condition. Tissue mRNA was extracted from kidney cortex (~40 mg) and cDNA was synthesized. Multiplex Real‐Time PCR was performed for transforming growth factors (TGF‐β1, TGF‐ β2 and TGF‐ β3). Housekeeping genes were Rpl19 and Cyclophilin‐A. Summary of Results Increased protein excretion in the Ob ad lib group was evident by 12 weeks of age compared to lean animals and increased further at 18 weeks. Food restriction beginning at 6 weeks of age prevented this from occurring. TGF‐β1 gene expression was elevated at 12 weeks of age in the Ob ad‐lib animals compared to lean or Ob‐Res. TGF‐β3 gene expression was increased at 6 weeks in the Ob ad lib compared to lean and remained elevated at 12 and 18 weeks of age compared to lean or Ob‐Res groups. The TGF‐β2 (not shown) had no elevated expression levels in any group. Conclusions Increased gene expression levels of TGF‐β1 and TGF‐ β3 suggest a role for these growth factors early in the development of obesity‐linked kidney disease, a conclusion strengthened by the complete prevention of these changes by food restriction which also prevented proteinuria. Project funded by VA Merit Review award, Avera Research Institute, and Sanford School of Medicine of the University of South Dakota.