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Quantification of von Willebrand Factor and von Willebrand Factor Propeptide in Patients with End Stage Renal Disease
Author(s) -
Davis Rachael,
Bansal Vinod,
Litinas Evangelos,
Rahman Saudur,
Hoppensteadt Debra,
Conway Brooke,
Fareed Jawed
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1030.4
Subject(s) - von willebrand factor , protein precursor , medicine , hemodialysis , end stage renal disease , pathophysiology , endocrinology , gastroenterology , chemistry , platelet , biochemistry , gene
In End Stage Renal Disease (ESRD), systemic vascular changes contribute to thrombotic comorbidities. The purpose of this study was to characterize the role of vWF in these inflammatory changes. Plasma samples from 51 patients with clinically confirmed (ESRD) were collected prior to maintenance hemodialysis. A group of 50 normal individuals, both male and female, was included as control. Concentrations of von Willebrand Factor (vWF) and von Willebrand Factor Propeptide (vWFpp) were quantified using the GTI® vWF & Propeptide Assay. Compared to controls, the levels of vWF and vWFpp were increased in patients with ESRD. These patients showed a 1.3 fold increase in vWF (mean 128 ± 69 U/dL, range 9.2 to 308) compared to control (mean 100 ± 60, range 22 to 332). Similarly, there was a 1.6 fold increase in vWFpp (mean 148 ± 92 U/dL, range 8.0 to 361) compared to control (mean 92 ± 23, range 60 to 163). However, the ratio of vWFpp:vWF (mean 1.2 ± 0.6, range 0.4 to 3.3) was unchanged compared to control. This study shows a previously uncharacterized increase in vWFpp and validates the up‐regulation of vWF in ESRD. The normal vWFpp:vWF ratio implies an increased production in vWF in response to endothelial damage without any apparent down‐regulation of ADAMTS‐13 mediated degredation of vWF. Further studies are required to assess the role of ADAMTS‐13 in ESRD to better understand the interplay of all these components.