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Nephrolithiasis in the Wpk/+ Rats
Author(s) -
Gattone Vincent,
Williams James,
Bledsoe Sharon,
EgglestonGulyas Tracy,
Miller Caroline,
Moe Sharon,
Evan Andrew
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1030.2
Subject(s) - kidney stones , kidney stone disease , kidney , calcification , tamm–horsfall protein , renal pelvis , anatomy , pathology , medicine , kidney disease
Nephrolithiasis affects over 10% of Americans and 10–15% of stones are calcium phosphate (CaP). CaP stone disease is poorly understood. Male Brown Norway Wpk/+ rats (12–18 months old, heterozygote carriers of a mutation for Meckel Syndrome) develop renal calcium deposits and pelvic stones. We evaluated kidney and stone morphologies from old BN‐Wpk/+ rats; kidneys were evaluated by light microscopy, microCT and infrared spectroscopy (FT‐IR). Renal calcification was commonly associated with hypertrophic papillary epithelium, and associated with the apical surface, not classic Randall's plaques. Additionally, kidney stones were found in pelvis and pelvic fornix in most rats, some of which were adherent to the papillae while others were free floating. Micro CT analysis of adherent kidney stones identified the attachment site surrounded by multiple laminations, suggesting slow growth over time—similar to human kidney stones. FT‐IR showed amorphous CaP as the primary mineral component. Rodent models of spontaneous kidney stone disease are few; this model is linked to a gene mutation and therefore could provide insight into the pathogenesis of CaP nephrolithiasis.