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Rapid and consistent porcine model for chronic total occlusions in the peripheral vasculature
Author(s) -
Tunev Stefan,
Caceres Alejandra,
Melder Robert,
Wilcox Josiah
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1028.6
Subject(s) - bone decalcification , calcification , medicine , context (archaeology) , tunica media , bone formation , pathology , anatomy , smooth muscle , biology , paleontology
Development and testing of treatment options for chronic total occlusions (CTO) still remains one of the last frontiers of vascular interventions. The lack of a consistent CTO animal model is one of the hurdles precluding efficient treatment advances in this field. We describe here a porcine model of a calcified CTO achieved by an intravascular delivery of de‐mineralized bone matrix (DBM) products in the femoral arteries. DBM is essentially de‐mineralized allograft bone particles with osteoinductive properties. In this study, the DBM material was delivered to each vessel via a guide catheter based approach. At 28 days, each treated vessel was evaluated angiographically, the animals were sacrificed and the treated vessels segments were assessed histopathologically. CTO was detected in 73% of the treated vessels. Morphologically, the CTO lesions were characterized by a mixed tissue growth composed of well vascularized collagenous matrix, mineralized and un‐mineralized bone fragments and variable mononuclear inflammation. This tissue reaction appeared well adhered to the vascular tunica media. Vascular channels lined by endothelium were present in vessels with incomplete chronic occlusion. This animal model can provide a platform for 1) development and testing of new CTO crossing devices, 2) evaluation of stents in calcified vessels 3) identification and evaluation of pharmacological entities in context of calcification and 4) development and evaluation of decalcification treatments.