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Oxygen transport from air to cell: The impact of age
Author(s) -
Amann Markus,
Wray Walter,
Wary Claire,
Monnet Aurelien,
Carlier Pierre G.,
Richardson Russell S.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1026.16
Subject(s) - myoglobin , cardiology , medicine , skeletal muscle , oxygen transport , chemistry , basal (medicine) , oxygen , biochemistry , organic chemistry , insulin
To evaluate potential age‐related differences in the O2 transport from air to skeletal muscle at rest, we studied 14 young (25 ± 2) and 13 old (71 ± 3) healthy males. Femoral venous and arterial catheterization was combined with the measurement of femoral blood flow (QL) (ultrasound Doppler) to quantify single leg O2 delivery and O2 consumption (VO2). Proton nuclear magnetic resonance spectroscopy was used to determine basal myoglobin desaturation (deoxy‐Mb) and intracellular O2 pressure (PiO2) was calculated using an O2/Mb half‐saturation of 3.2 mmHg. Utilizing these data, a Bohr integration technique was used to calculate muscle O2 diffusion capacity (DmO2). Despite a similar leg muscle mass (~9.5 l) and basal leg VO2 (~24 ml/min), QL and O2 delivery were significantly lower in the old vs young subjects (270 vs 324 ml/min and 52 vs 63 ml/min, respectively). However, neither myoglobin concentration (~0.3 mM) nor the average basal deoxy‐Mb signal (~11%) were different between the two age groups. Consequently, the calculated PiO2 was nearly identical in the old and young subjects (~32 mmHg) and calculated resting DmO2 was 28% greater in the elderly. Our data confirm previous reports of attenuated QL and O2 delivery with age, but suggest that, as leg VO2 and PiO2 were similar between young and old, aging improves O2 diffusion from capillary to myocyte at rest.