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Hypertension in response to agonistic autoantibodies to the angiotensin II type I receptor (AT1‐AA): role of reactive oxygen species (ROS)
Author(s) -
LaMarca Babbette,
Parrish Marc,
Weimer Abram,
Arany Marrietta,
Cockrell Kathy,
Dechend Ralf
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1025.6
Subject(s) - angiotensin ii receptor type 1 , angiotensin ii , reactive oxygen species , medicine , endocrinology , oxidative stress , preeclampsia , chemistry , telmisartan , superoxide dismutase , placenta , nadph oxidase , receptor , pharmacology , blood pressure , fetus , biology , biochemistry , pregnancy , genetics
AT1‐AA and ROS are implicated in hypertension in preeclamptic women. Administration of purified rat AT1‐AA significantly increases mean arterial pressure (MAP) and placental ROS in normal pregnant rats. The objective of this study was to determine the role of ROS in mediating AT1‐AA induced hypertension during pregnancy. To achieve this goal, MAP and ROS were analyzed in AT1‐AA induced hypertensive pregnant rats in the presence and absence of a superoxide dismutase mimetic, Tempol. Rat AT1‐AA (1:50) was infused intraperitoneally via osmotic minipumps and Tempol (30mg/kg/day)was delivered in drinking water of pregnant rats beginning on day 12 of gestation. On day 19 MAP was analyzed and placentas were collected for ROS analysis via lucigenin chemiluminescence technique. MAP was 101 + 2 in NP rats and 116 + 2 mmHg AT1‐AA rats (p=0.002). Placental basal and NADPH oxidase stimulated ROS was 29 +/− 6 and 92 +/− 10 RLUs in NP rats. These levels increased to 159 +/− 29 (P<0.01) and 287 +/− 60 RLUs (P<0.001) in AT1‐AA rats. MAP in AT1‐AA + Tempol rats was 109 +/−3 vs 109+/−2 mmHg in Tempol treated controls. Administration of Tempol decreased ROS in AT1‐AA treated rats (121+/−13; 262 +/−21 RLUs) compared to Tempol treated controls (69 +/−24; 141 +/− 33 RLUs) AT1‐AA induced hypertension is associated with placental placenta oxidative stress and is attenuated with administration of an SOD mimetic