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Sham rehydration contributes to increased Fos staining in the hindbrain after water deprivation in the rat
Author(s) -
Little Joel Turner,
Knight W David,
Ji Lisa Liping,
Gottlieb Helmut,
Cunningham J Thomas
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1025.16
Subject(s) - staining , saline , medicine , endocrinology , pathology
This experiment tested the role of the oropharyngeal and gastric afferents on Fos staining in the hindbrain of dehydrated and rehydrated rats instrumented with gastric fistulae. Rats were divided into control (CON) or 48 hour water deprivation (WD) treatments. WD rats were further subdivided to include 3 rehydration treatments: 2 hour rehydration with fistulae closed (RHC), fistulae open (RHO), or rehydration with isotonic saline (RHS). An open fistula facilitates sham rehydration while closed fistulae allow for complete ingestion; WD and RHS rats also had open fistulae. WD increased osmolality and only RHC treatment reduced osmolality (CON: 296±2 WD: 307±2 RHC: 278±2 RHO: 305±1 RHS: 308±3). Hindbrains were processed for fos and the nucleus of the solitary tract (NTS) for each rat was examined. Water deprivation modestly increased Fos in the NTS (CON: 7±2; WD: 15±3). Rehydration significantly increased Fos staining in both RHO (37±6) and RHC (35±6) rats compared to CON and WD rats. In RHS rats, Fos staining was only slightly elevated compared to WD rats (23±4) and significantly less than RHC and RHO rats. Fos staining in the NTS associated with rehydration appears to be mediated by visceral afferents activated during sham rehydration and possibly independent of the solute concentration of the solution ingested. Supported by NIH R01 HL62579.