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Hyaluronic acid attenuates expression of HIF‐1 alpha and iNOS in experimental rheumatoid arthritis
Author(s) -
Chang PeiLin,
Hsieh YuehLing,
Tu PinWen,
Chou LiWei,
Ou HsiuChung
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1022.11
Subject(s) - hyaluronic acid , medicine , rheumatoid arthritis , nitric oxide synthase , arthritis , hypoxia (environmental) , intraperitoneal injection , nitric oxide , immunohistochemistry , endocrinology , immunology , chemistry , oxygen , anatomy , organic chemistry
Rheumatoid arthritis (RA) is known to be associated with hypoxia‐related joint diseases. The role of hyaluronic acid (HA) on management of RA and its possible anti‐hypoxic abilities are not well known. The purpose of this study is to assess the effects of intra‐articular injection of HA on expression of hypoxia‐inducible factor‐1 alpha (HIF‐1α) and inducible nitric oxide synthase (iNOS) in adjuvant‐induced arthritis rats. Adult SD rats with complete Freund's adjuvant‐induced arthritis (CIA) were subjected to short‐term (day 1) or long‐term (days 1, 5, 10) intra‐articular HA treatments. Buffer‐treated CIA rats served as arthritis controls. Effects of HA in CIA animals were determined by von Frey filament test, joint circumference assessment and HIF‐1α and iNOS immunohistochemistry. The extent and grade of joint swelling and pain behavior in the long‐term HA injection rats were less severe than those of short‐term HA injection and control rats. HA significantly suppressed expressions of HIF‐1α and iNOS in cartilage and synovium after long‐term HA injection. Results indicated that presence of hypoxia in inflamed joints of this CIA model is suppressed by HA dose‐dependent manner. Therefore, down‐regulation of HIF‐1α and iNOS might be important mechanisms of intra‐articular injection of HA in the treatment of RA. The study is supported partially by a grant from the China Medical University (CMU98‐S‐10) of Taiwan.