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GLUTAMATE SUPPRESSES HELICOBACTER PYLORI‐INDUCED GASTRIC ATROPHY ‐MECHANISMS OF CHIEF CELL PROTECTION
Author(s) -
Nakamura Eiji,
Hasumura Mai,
Gabriel Ana San,
Tashima Kimihito,
Amagase Kikuko,
Uneyama Hisayuki,
Takeuchi Koji,
Torii Kunio
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.102.8
Subject(s) - glutamine , helicobacter pylori , gastric chief cell , glutamine synthetase , viability assay , glutamate receptor , atrophy , glutathione , biochemistry , secretion , chemistry , cell damage , medicine , biology , cell , amino acid , gastric mucosa , stomach , enzyme , receptor
Luminal free glutamate (Glu) induces gastroduodenal mucus secretion, suggesting the protective roles of dietary free Glu during digestion process. We have previously reported that Glu supplemented in the diet reduces Helicobacter pylori (HP)‐induced gastric mucosal damages in gerbils. Surprisingly, gastric atrophy (i.e. the loss of pepsinogen‐secreting chief cells and acid‐secreting parietal cells) was also reduced by Glu supplementation. To clarify the mechanisms how Glu reduces gastric atrophy against HP, we investigated the direct protective role of Glu on the cell viability of cultured chief cells against ammonia, a known HP toxin. NH 4 Cl (an ammonia donor) significantly reduced cell viability. Glu, glutamine, asparagine and aspartate all protected cultured chief cells against NH 4 Cl. Pharmacological analysis revealed that Glu synthetase inhibitors, L‐methionine sulfoximine and L‐2‐amino adipic acid protected chief cells against NH 4 Cl‐induced cell death. In contrast, glutathione synthetase inhibitor, L‐buthionine sulfoximine had no effect on Glu‐induced chief cell protection against NH 4 Cl. These results suggest that Glu reduces HP‐induced gastric atrophy partly via glutamine synthetase‐mediated pathway. Thus, we propose that free Glu in the diet would contribute to the protection from gastric atrophy against HP in daily life.