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Prevention of Mycobacterium avium subsp. paratuberculosis (MAP) infection in BALB/c mice by feeding probiotic Lactobacillus acidophilus NP‐51®
Author(s) -
Osman Mohamed A,
Stabel Judy R,
Hostetter Jesse M,
Nettleton Daniel S,
Beitz Donald C
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.102.5
Subject(s) - mycobacterium avium subspecies paratuberculosis , paratuberculosis , microbiology and biotechnology , biology , probiotic , lactobacillus acidophilus , mycobacterium , splenocyte , mycobacterium avium subsp. paratuberculosis , lactobacillus , immunology , bacteria , immune system , food science , fermentation , genetics
The objective of this study was to examine effects of feeding Lactobacillus acidophilus strain NP51 to mice challenged with Mycobacterium avium subspecies paratuberculosis ( MAP ). Mice were randomized to ten treatment groups; sentinels, control, heat‐killed MAP, viable MAP, heat‐killed NP51, viable NP51, heat‐killed NP51 plus heat‐killed MAP, heat‐killed NP51 plus viable‐MAP, viable‐NP51 plus heat‐killed MAP, viable‐NP51 plus viable‐MAP. Mice were fed 1 × 10 6 CFU of NP51· mice −1 · day −1 . On day 45, mice were challenged with 1 × 10 8 CFU of MAP intraperitonealy. We hypothesized that feeding NP51would increase Th‐1 responses and decrease progression of Johne's disease in mice. Ten mice from each group were euthanized on days 45, 90, 135 and 180. Supernatante from in vitro splenocyte culture was examined for IFN‐γ production. Feeding Heat‐killed NP51 to heat‐killed MAP‐ or viable MAP‐infected mice increased interferon ( IFN )‐γ by 1.4‐ and 4‐fold (44.32 and 127.14 vs. 31.69 pg/mL, respectively) over that of heat‐killed MAP‐infected control. Similarly, feeding viable‐NP51 to heat‐killed MAP‐ or viable MAP‐infected mice increased IFN‐γ by 3‐ and 4‐ fold (98.6 and 130.68 vs. 31.69, respectively) over that of heat‐killed MAP‐infected control. These data suggest that feeding NP51 can simulate IFN‐γ production and prevent progression of Johne's disease in mice. Funding source: Nutrition Physiology Company, Guymon, OK. Grant Funding Source : Nutrition Physiology Company